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T.K.S. Kumar CHEM 235 |
Degrees:
Ph.D. Osmania University, Hyderabad, India - 1993
Research Interests:
protein structure, dynamics, interaction and folding
Research:
- Determination of three-dimensional solution structures of proteins using multidimensional NMR spectroscopy.
- Characterization of protein-protein interactions using a variety of biophysical techniques such as circular dichroism, fluorescence spectroscopy, Isothermal titration calorimetry, Infrared spectroscopy and NMR spectroscopy.
- Investigation of the backbone dynamics of proteins based on 15N-and 13C-relaxation measurements using NMR spectroscopy.
- Rational design of drugs by SAR by NMR and molecular modeling techniques.
- Characterization of protein-folding/unfolding pathways of beta-sheet proteins and membrane proteins.
- Development of new pulse sequences for multidimensional NMR spectroscopy.
- Understanding the specificity of protein aggregation. Characterization of amyloid-like fibrils and development of new experimental strategies to recover recombinant proteins from inclusion bodies
Publications/Presentations:
87. Sivaraja, V., T. K. S. Kumar, D. Rajalingam, I. Graziani, I. Prudovsky and C. Yu. Copper binding affinity of S100A13, a key component of FGF-1 Non-Classical Copper-Dependent Release Complex. (2006) Biophys. J. (in press)
86. Hseih, H. C., T. K. S. Kumar, T. Sivaraman and C. Yu. Refolding of a Small All Beta-Sheet Protein Proceeds with Accumulation of Kinetic Intermediates (2006) Arch. Biochem. Biophys., 447, 147.
85. Kathir, K. M., T. K. S. Kumar and C. Yu. Understanding the Anti-Mitogenic Activity of Suramin (2006) Biochemistry, 45, 899.
84. Sivaraja, V., T. K. S. Kumar, P. S. T. Leena, A. Chang, C. Vidya, R. Goforth, D. Rajalingam, A. Kannan, J. L.Ye, J. Chou, R. Henry and C. Yu. Three-dimensional Solution Structures of the Chromo Domains of cpSRP43. (2005) J. Biol. Chem. 280, 41465.
83. Hung, K. W., T. K. S. Kumar, K. M. Kathir, P. Xu, F. Ni, H. H. Ji, M. C. Chen, C. C. Yang, F. P. Lin and C. Yu. Solution Structure of the Ligand-Binding Domain of the Fibroblast Growth Factor Receptor – Role of Heparin in the Activation of the Receptor. (2005) Biochemistry, 44, 15787.
82. Rajalingam, D., T. K. S. Kumar and C. Yu. C2A Domain of Synaptotagmin Exhibits High Binding Affinity for Copper – Implications in the Formation of the Multiprotein Release Complex. (2005) Biochemistry, 44, 14431.
81. Rajalingam, D., T. K. S. Kumar, R. Soldi, I Graziani, I. Prudovsky and C. Yu. Molecular Mechanism of Inhibition of the Non-Classical FGF-1 Export (2005) Biochemistry, 44, 15472.
80. Sivaraja, V., T. K. S. Kumar, I. Prudovsky and C. Yu Three-Dimensional Solution Structure of a Unique S100 Protein. (2005) Biochem. Biophys. Res. Commun., 335, 1140.
79. Kathir, K. M., T. K. S. Kumar, D. Rajalingam and C. Yu. Time-dependent Changes in the Denatured State(s) Affect Folding Mechanism of an All Beta-Sheet Protein. (2005) J. Biol. Chem. 280. 29682.
78. Sivaraja, V.; T. K. S. Kumar, and C. Yu. Resonance Assignments for Mouse S100A13 (2005)Assignment of 1H, 13C, and 15N Resonances in S100A13 (2005) J. Biomol. NMR, 32, 257.
77. Jayachithra, K., T. K.S. Kumar, T. J. Lu, C. Yu, C. and D. H. Chin Cold Instability of Aponeocarzinostatin and its stabilization by Labile Chromophore (2005) Biophys. J., 88, 4252.
76. Hsieh, H. C., T. K. S. Kumar, Chiu, C. C and C. Yu. Equilibrium Unfolding of an Oligomeric Protein Involves the Formation of a Multimeric Intermediate. (2005) Biochem. Biophys. Res. Commun., 326, 108.
75. Kumar, T. K. S. and C. Yu. Monitoring Protein Folding at Atomic Resolution. (2004) Acc. Chem. Res., 37, 929.
74. Hung, K. W., T. K. S. Kumar and C. Yu. 1H, 13C and 15N chemical shift assignments of the D2 domain of the fibroblast growth factor receptor. (2004) J. Biomolec. NMR., 30, 99.
73. Hsieh, H. C., T. K. S. Kumar and C. Yu. Cloning, overexpression, and characterization of cobrotoxin. (2004) Biochem. Biophys. Res. Commun., 320, 1374.
72. Hung, K. W., T. K. S. Kumar and C. Yu. Molecular cloning, overexpression, and characterization of the ligand-binding D2 domain of fibroblast growth factor receptor. (2004) Biochem. Biophys. Res. Commun., 317, 253.
71. Srisailam, S., T. K. S. Kumar, D. Rajalingam, K. M. Kathir, H. S. Sheu, F. J. Jan, P. S. Chao and C. Yu. Amyloid-like formation in an all beta sheet protein. Partially Structured State(s) is a precursor for Fibril Formation (2003) J. Biol. Chem. 278, 17701.
70. Srimathi, T., T. K. S. Kumar,, K. M. Kathir, Y. H. Chi, S. Srisailam, W. Y. Lin and C. Yu. Structurally Homologous all Beta-Barrel Proteins Adopt Different Folding Mechanisms (2003) Biophys. J., 85, 459.
69. Chi, Y. H., T. K. S. Kumar, K. M. Kathir, D. H. Lin, G. Zhu, I. M. Chiu and C. Yu . Investigation of the Structural Stability of the Acidic Fibroblast Growth Factor by Hydrogen-Deuterium Exchange (2002) Biochemistry, 41, 13350.
68. Srimathi, T., T. K. S. Kumar, Y. H. Chi, I. M. Chiu and C. Yu. Characterization of the Structure and Dynamics of a Near-Native Intermediate in the Unfolding Pathway of an All Beta-Sheet J. Biol. Chem. (2002) 277, 47507.
67. Arunkumar, A. I., S. Srisailam, T. K. S. Kumar, K. M. Kathir, H. M. Wang, G. G. Chang and C. Yu. Three-Dimensional Structure of an Acidic Fibroblast Growth Factor from Notopthalmus viridescence (2002) J. Biol. Chem. 277, 46424.
66. Chi, Y. H., T. K. S. Kumar, I. M. Chiu and C. Yu . Identification of Rare Partially Unfolded States in Equilibrium with Native Conformation in an All Beta-Barrel Protein (2002) J. Biol. Chem., 277, 34941 -34948
65. Srinivasan, K. N.., V. Sivaraja, J. Huys, T. Sasaki, B. Cheng, T. K. S. Kumar, K. Sato, J. Tytgat, C. Yu, C. S. Brian Chia, S. Ranganathan, J. H. Bowie, R. M. Kini and Gopalakrishnakone. (2002) kappa-Hefutoxins, a novel class of potassium channel toxins with unique structure and activity. J. Biol. Chem., 277, 30040
64. Srisailam, S., H. M. Wang, T. K. S. Kumar, D. Rajalingam, V. Sivaraja and C. Yu (2002) Amyloid-like Formation in an All beta-Barrel Protein Involves the Formation of Partially Structured Intermediate(s). J. Biol. Chem., 277, 19027
63. Kumar, T. K. S., S. Srisailam, R. R. Vethanayagam and C. Yu Understanding the Structure - Function Relationship of Snake Venom Cardiotoxins. (2002) in Perspectives in Molecular Toxinology [Ed. A. Menez] pp. 315 – 325, John Willey & Sons, Ltd, Sussex, England.
62. Arunkumar, A. I., T. K. S. Kumar, K. M. Kathir, S. Srisailam, H. M. Wang, P. S. T. Leena, Y. H. Chi, C. H. Wu, R. T. Wu, I. M. Chiu and C. Yu. Oligomerization of acidic fibroblast growth factor is not a prerequisite for its cell proliferation activity (2002) Protein Science, 11, 1050.
61. Srisailam, S., T. K. S. Kumar, T. Srimathi and C. Yu. Influence of Backbone Conformation on Protein Aggregation, (2002) J. Am. Chem. Soc., 124, 1884.
60. Kumar, T. K. S. and C. Yu Cardiotoxins – Structure and Pharmacology (2002) in Encyclopedia of Molecular Medicine (Edited by T. E. Creighton) pp. 465 – 467, Wiley & Sons, New York, USA
59. Srisailam, S., T. K. S. Kumar, A. I. Arunkumar , K. W. Leung, C. Yu and Chen, H. M. Crumpled Structure of the Custom Hydrophobic Lytic Peptide Cecropin B3: Implication of the Flip-Flop Lysis on Lipid Layers. (2001) Eur. J. Biochemistry, 268, 4278
58. Samuel, D., T. K.S. Kumar, K. Balamurugan, W. Y. Lin, D. H. Chin and C. Yu. Structural Events During the Refolding of an All beta-sheet Protein. (2001) J. Biol. Chem., 276, 4134-4141
57.Chi, Y. H., T. K. S. Kumar, I. M. Chiu and C. Yu. Thermodynamic Characterization of the Human Acidic Fibroblast Growth Factor – Evidence for Cold Denaturation. (2001) Biochemistry, 40, 7746.
56. Chi, Y. H., T. K. S. Kumar, I. M. Chiu and C. Yu 15N NMR Studies of Free and Ligand bound Human Acidic Fibroblast Growth Factor (2000) J. Biol. Chem. 275, 39444.
55. Ganesh, G., T. K. S. Kumar, S. Pandian and C. Yu. Rapid staining of proteins on polyacrylamide gels and nitrocellulose membranes using a mixture of fluorescent dyes (2000) J. Biophys. Biochem. Methods, 46, 31.
54. Kumar, T. K. S., T. Sivaraman, D. Samuel, S. Srisailam, G. Ganesh, H. Hui-chu, K. W. Hung, A. I. Arunkumar and C. Yu (2000) Protein folding and beta-Sheet proteins. J. Chinese Chem. Soc., 49, 1009
53. Samuel, D., T. K. S. Kumar, T. Srimathi, H. C. Hsieh and C. Yu. Identification and Characterization of an Equilibrium Intermediate in the Unfolding Pathway of an all (-barrel Protein. . (2000) J. Biol. Chem., 275, 34968.
52. Arunkumar, A. I., S. Srisailam, T. K. S. Kumar, K. M. Kathir, C. L. Peng, C. Chen, I. M. Chiu and C. Yu 1H, 13C and 15N chemical shift assignments of the acidic fibroblast growth factor from Notopthalamus Viridescens. (2000) J. Biomolec. NMR., 17, 279.
51. Sivaraman, T., T. K.S. Kumar, K. W. Hung and C. Yu. Comparison of the structural stability of two homologous toxins isolated from the Taiwan cobra (Naja naja atra) venom (2000) Biochemistry, 39, 8705.
50. Jayaraman, G., T. K. S. Kumar, C. C. Tsai, S. Srisailam, S. L. Chou, C. L. Ho and C. Yu. Elucidation of the solution structure of cardiotoxin analogue V from the Taiwan cobra (Naja naja atra) - Identification of structural features important for the lethal action of snake venom cardiotoxins. (2000) Protein Science., 9, 637.
49. Samuel, D., T. K. S. Kumar, G. Ganesh, G. Jayaraman, P. W. Yang, M. M. Chang, V. D. Trivedi, S. L. Wang, K. W. Hwang, D. K. Chang and C. Yu. Proline inhibits protein aggregation during protein refolding (2000) Protein Science, 9, 356.