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Julie Stenken CHEM 247 |
Degrees:
B.S. Chemistry, University of Akron, 1990
Ph.D. Bioanalytical Chemistry, University of Kansas, 1995
J. William Fulbright Fellow, The Karolinska Institute, Stockholm Sweden, 1994-1995
Postdoctoral Fellow, University of Kansas Medical School, Kansas City, KS, 1995-1996
Affiliations:
American Association for the Advancement of Science
American Chemical Society
Society for Biomaterials
Society for Neuroscience
Society for Free Radical Biology and Medicine
Research:
Our interdisciplinary research involves the areas of bioanalytical chemistry, bioengineering, biomaterials, and signal transduction and focuses on the development and improvement of analytical methods for in vivo measurements. A primary aim in the Stenken group is to understand the inflammatory response caused by macrophages to implanted foreign materials. Understanding the underlying biochemistry that occurs at the site of an implanted biomaterial is important in a wide range of clinical contexts — from reconstructive surgery to implantable glucose sensors. Many of the problems that have been encountered in the development of in vivo sensors have been due to the lack of understanding of the host response to implanted materials. To access the microenvironment of the foreign body response to a biomaterial, we use microdialysis sampling. A wide range of chromatographic, immunochemical, and spectroscopic analysis methods are used and developed by our research group for analyte detection in the low microliter volume dialysates.
Current projects include collection and detection of the soluble messenger proteins (cytokines) during the inflammatory response; creation and mathematical modeling of microdialysis enhanced mass transport techniques; in situ detection of matrix metalloproteinases; calibration of microdialysis sampling devices during protein collection; and creation of improved in vivo sampling devices.
Publications/Presentations:
Selected Publications
D.D. Cunningham and J.A. Stenken (editors) In Vivo Glucose Sensing. John Wiley & Sons. 2009. In the press.
H.J. Fletcher and J.A. Stenken. An in vitro comparison of microdialysis relative recovery of met- and leu-enkephalin using cyclodextrins and antibodies as affinity agents. Analytica Chimica Acta. 2008, 620, 170-175.
X. Wang, D. J. Loegering, M.R. Lennartz and J. A. Stenken. Multiplexed cytokine detection of interstitial fluid collected from polymeric hollow tube implants. Cytokine, 2008, 43(1) 15-19.
Y. Wang, D.V. Zagorevski, and J. A. Stenken, In situ and Multi-Substrate Detection of Elastase Enzymatic Activity External to Microdialysis Sampling Probes using LC-ESI-MS, Analytical Chemistry, 2008, 80, 2050-2057.
A. Gupta, H. Denver, A.H. Hirsa,J. Stenken,D.-A. Borca-Tasciuc. Localized, low-voltage electroosmotic pumping across nanoporous membranes. Applied Physics Letters, 2007, 91: 09410/1-09410/3.
L.Sun , J.A. Stenken, A.Y. Yang, J. J. Zhao, and D.G. Musson . An in vitro microdialysis methodology to study 11β-hydroxysteroid dehydrogenase type 1 enzyme activity in liver microsomes. Analytical Biochemistry, 2007, 370, 26-37.
X. Wang, M.R. Lennartz, D.J. Loegering, and J.A. Stenken. Interleukin-6 Collection through Long-Term Implanted Microdialysis Sampling Probes in Rat Subcutaneous Space. Analytical Chemistry, 2007, 79, 1816-1824.
X Mou and J.A. Stenken. Microdialysis sampling extraction efficiency of 2-deoxyglucose: The role of macrophages in vitro and in vivo. Analytical Chemistry. 2006, 78, 7778-7784.
J. Duo, H. Fletcher, and J.A. Stenken. Natural and synthetic affinity agents as microdialysis sampling mass transport enhancers. Current progress and future perspectives. Biosensors and Bioelectronics: Synthetic Receptors. 2006, 22, 449-457.
J.A. Stenken. Microdialysis Sampling, Encyclopedia of Medical Devices and Instrumentation, 2nd Edition, Volume 4. John G. Webster (Editor). John Wiley & Sons, Inc. Hoboken, NJ. 2006 pp. 400-420.
X. Ao and J. A. Stenken. Microdialysis sampling of cytokines. Methods. 2006, 38, 331-341
R. Chen, J.T. Warden, and J.A. Stenken, Microdialysis sampling coupled with ESR spin trapping for superoxide radical detection in microliter sample volumes. Analytical Chemistry, 2004, 76, 4734-4740.
J.A. Stenken, W. M. Reichert, and B. Klitzman. Magnetic resonance imaging of a tissue/implanted device
biointerface using in vivo microdialysis sampling. Analytical Chemistry, 2002, 74, 4849-4854.
J.A. Stenken. Methods and issues in microdialysis calibration. Analytica Chimica Acta 1999, 379, 337-358.