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Julie Stenken CHEM 247 |
Degrees:
B.S. Chemistry, University of Akron, 1990
Ph.D. Bioanalytical Chemistry, University of Kansas, 1995
J. William Fulbright Fellow, The Karolinska Institute, Stockholm Sweden, 1994-1995
Postdoctoral Fellow, University of Kansas Medical School, Kansas City, KS, 1995-1996
Affiliations:
American Association for the Advancement of Science
American Chemical Society
Society for Biomaterials
Society for Neuroscience
Society for Free Radical Biology and Medicine
Research:
Our interdisciplinary research involves the areas of bioanalytical chemistry, bioengineering, biomaterials, and signal transduction and focuses on the development and improvement of analytical methods for in vivo measurements. A primary aim in the Stenken group is to understand the inflammatory response caused by macrophages to implanted foreign materials. Understanding the underlying biochemistry that occurs at the site of an implanted biomaterial is important in a wide range of clinical contexts — from reconstructive surgery to implantable glucose sensors. Many of the problems that have been encountered in the development of in vivo sensors have been due to the lack of understanding of the host response to implanted materials. To access the microenvironment of the foreign body response to a biomaterial, we use microdialysis sampling. A wide range of chromatographic, immunochemical, and spectroscopic analysis methods are used and developed by our research group for analyte detection in the low microliter volume dialysates.
Current projects include collection and detection of the soluble messenger proteins (cytokines) during the inflammatory response; creation and mathematical modeling of microdialysis enhanced mass transport techniques; in situ detection of matrix metalloproteinases; calibration of microdialysis sampling devices during protein collection; and creation of improved in vivo sampling devices.
Publications/Presentations:
X. Wang, M.R. Lennartz, D.J. Loegering, and J.A. Stenken. Interleukin-6 Collection through Long-Term Implanted Microdialysis Sampling Probes in Rat Subcutaneous Space. Analytical Chemistry, 2007, 79, 1816-1824.
J.A. Stenken. (2006) Microdialysis Sampling, Encyclopedia of Medical Devices and Instrumentation, 2nd Edition, Volume 4. John G. Webster (Editor). John Wiley & Sons, Inc. Hoboken, NJ. pp. 400-420.
X Mou and J.A. Stenken. Microdialysis sampling extraction efficiency of 2-deoxyglucose: The role of macrophages in vitro and in vivo. Analytical Chemistry. 2006, 78, 7778-7784.
X. Wang and J.A. Stenken, Microdialysis sampling membrane performance during in vitro macromolecule collection, Analytical Chemistry. 2006, 78, 6026-6034.
X. Ao and J. A. Stenken. Microdialysis sampling of cytokines. Methods. 2006, 38, 331-341. (Invited Review)
X. Ao, R.F.Rotundo, D.J. Loegering, and J.A. Stenken. In vivo microdialysis sampling of cytokines produced in mice given bacterial lipopolysaccharide. The Journal of Microbiological Methods, 2005, 62, 327-336.
R. Chen, J.T. Warden, and J.A. Stenken, Microdialysis sampling combined with electron spin resonance for superoxide radical detection in microliter samples. Analytical Chemistry, 2004, 76, 4734-4740.
X. Ao, T.J. Sellati and J.A. Stenken. Enhanced microdialysis relative recovery of inflammatory cytokines using antibody-coated microspheres analyzed by flow cytometry. Analytical Chemistry, 2004, 76, 3777-3784.